scRNAseq data generation
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- Eukaryotic cell composition
- Cell behavior / function
- Central dogma

  + DNA
  + RNA
  + Protein

- Why RNA-seq?
- Applications of scRNSeq

  + Identity, states, atlases
  + Spatial information
  + Dynamics via TI
  + Lineage potency

- Examples

  + Tang et al Cell Stem Cell 2010
  + Kumar et al Nature 2014

- Brief history of scRNAseq
- DropSeq

  + overview
  + barcoding
  + bead & cell capture
  + cDNA
  + Sequence anatomy
  + doublets
  + cell cycle
  + Retina cell type census
  + How many cells are needed?

- Homework 1

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