RNA Velocity

• Guest Lecture by Kathleen Noller

• Review of trajectory inference (TI)

• What is RNA Velocity (RV)?

• RV vs TI

• Splicing and transcription

• Measuring spliced vs unspliced transcripts

• RV model & assumptions

• ds/du = u - gamma * s

• u = unspliced mRNA

• s = spliced RNA

• gamma = degradation rate

• assumes constant splicing rate

• Phase portrait

• Example: bulk RNAseq and cell cycle

• Example: scRNAseq Schwann progenitors

• Example: scRNAseq mouse hippocampus

• Splicing and degradation rates

• scVelo

• Dynamo

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