scRNAseq data generation

• Eukaryotic cell composition

• Cell behavior / function

• Central dogma

  • DNA

  • RNA

  • Protein

• Why RNA-seq?

• Applications of scRNSeq

  • Identity, states, atlases

  • Spatial information

  • Dynamics via TI

  • Lineage potency

• Examples

  • Tang et al Cell Stem Cell 2010

  • Kumar et al Nature 2014

• Brief history of scRNAseq

• DropSeq

  • overview

  • barcoding

  • bead & cell capture

  • cDNA

  • Sequence anatomy

  • doublets

  • cell cycle

  • Retina cell type census

  • How many cells are needed?

• Homework 1

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