Cell fate engineering

• Terminology:

  • Direct programming

  • Directed differentiation

  • Reprogramming

  • Direct conversion

• How TF pools are defined and refined

• Computational means to define factor cocktails

• Expression alone (D’Alessio et al)

• Expression + GRN (Cahan et al 2014; Rackham et al 2016; Hartmann 2018)

• Comparison of directed differentiation and direct programming with scRNAseq (Briggs et al 2017)

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